Thermo Scientific Human genomic DNA is enzymatically methylated with CpG Methyltransferase (M.SssI). Genomic DNA was isolated from blood samples from healthy male (HIV, HBV, and HCV negative) individuals. Isolated genomic DNA was treated with CpG Methyltransferase, repurified, and dissolved in TE buffer. ≥ 98% CpG methylation level confirmed by bisulfite sequencing.
Highlights
• Pure—DNA free from inhibitors, proteins and RNA
• Convenient—ready to use 100 ng/μL concentration
• Compatible with downstream applications
• Complete methylation of cytosines in CpG dinucleotides
Applications
As controls in the following:
• Bisulfite sequencing
• Combined Bisulfite Restriction Analysis (COBRA)
• Methylation-sensitive Single-Nucleotide Primer Extension (Ms-SNuPE)
Code | Description |
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SD1131 | Catalog Number: SD1131 |