MEGAclear™ Transcription Clean-Up Kit (Invitrogen™)

The MEGAclear™ Kit is designed for the quick cleanup of any large-scale transcription reaction. The MEGAclear™ Kit efficiently separates RNA from unincorporated NTPs, enzymes, and buffer components. Advantages of the MEGAclear™ Kit:

• Purifies transcription reactions to produce high-quality RNA, free of unincorporated NTPs, enzymes, and buffer components
• High recovery from 1 ng to 500 μg of input
• Ideal for purification of aRNA amplification reactions, nonisotopically labeled RNA probes, and capped RNA transcripts for oocyte injections
• Quick protocol—purify RNA in less than 30 minutes

Using the MEGAclear™ Kit
The protocol is quick and easy. The transcription reaction is mixed with Binding Solution, passed over an RNA-binding filter, and washed; an elution step releases the purified RNA, which is now ready for any application requiring highly pure synthetic or in vitrotranscribed RNA. For RNA recovery of 70% or greater, single-stranded RNA should be >100 nt and double-stranded RNA should be >200 bp.