MicroSEQ™ ID Purification Combo Kit, with clean-up plates (Applied Biosystems™)

This MicroSEQ™ ID Purification Combo Kit includes reagents for post-PCR and post-sequencing steps in the MicroSEQ™ ID microbial identification workflow. It contains 3 tubes of ExoSAP-IT™ Reagent for PCR clean-up and two 96-well MicroSEQ™ ID Sequencing Cleanup Plates.

ExoSAP-IT™ Reagent: this reagent is used for quick post-PCR clean-up for downstream applications such as sequencing. It allows for removal of unconsumed dNTPs and primers remaining in the PCR mix following amplification.

ExoSAP-IT™ Reagent can be used downstream of the following PCR amplification kits:
• MicroSEQ™ 500 16S rDNA PCR Kit
• MicroSEQ™ Full Gene 16S rDNA PCR Kit
• MicroSEQ™ D2 rDNA Fungal PCR Kit

MicroSEQ™ ID Sequencing Clean-up Plates: these plates are used for post-sequencing clean-up in the MicroSEQ™ ID workflow. The gel filtration matrix used in the microplate cartridges provide a convenient and simple way to remove dye terminators, dNTPs, and salts, as well as residual primers from cycle sequencing reactions. The end products can be directly used for sequencing on any Applied Biosystems™ genetic analyzers.

MicroSEQ™ ID Sequencing Cleanup Plates can be used downstream of the following sequencing kits:
• MicroSEQ™ 500 16S rDNA Sequencing Kit
• MicroSEQ™ Full Gene 16S rDNA Sequencing Kit
• MicroSEQ™ D2 rDNA Fungal Sequencing Kit