Oligofectamine™ Transfection Reagent (Invitrogen™)

Oligofectamine™ Transfection Reagent is a proprietary formulation for transfecting oligonucleotides and short interfering RNA (siRNA) into eukaryotic cells. Oligofectamine™ Transfection Reagent forms stable complexes with oligos, permitting efficient transfection into eukaryotic cells in a highly specific, yet nontoxic fashion. Oligofectamine™ Reagent is suitable for nuclear and cytoplasmic targets and transfects a wide variety of cell lines including CHO, HEK-293, NIH 3T3, and HeLa.

Using Oligofectamine™ Transfection Reagent
Oligofectamine™ Reagent is easy to use because it provides a simple and fast protocol. Just dilute Oligofectamine™ Reagent, mix with oligonucleotide, and add to your cells. Oligofectamine™ Reagent requires nanomolar quantities of antisense oligonucleotide, reducing the amount of valuable oligonucleotide needed by up to a thousand-fold. This makes it ideal for high-throughput applications. In addition, Oligofectamine™ Reagent has also been shown to work for siRNA transfections. We recommend this reagent when performing RNAi knockdown experiments in HeLa cells. Please visit RNAi Central for more information.