Oncomine™ Myeloid Assay GX v2 (Ion Torrent™)

The Ion Torrent Oncomine Myeloid Assay GX v2 is a comprehensive targeted next-generation sequencing (NGS) assay designed for sensitive detection of myeloid disorder-associated DNA mutations and RNA fusion transcripts in blood and bone marrow samples. This assay is compatible with the Genexus Integrated Sequencer, which performs library preparation, sequencing, analysis, and reporting in an automated sample-to-result workflow. Depending on the workflow, results can be obtained in as little as a single day.

Oncomine Myeloid Assay GX v2 features include:
• Comprehensive coverage of key DNA mutations and >700 fusion transcripts associated with myeloid disorders
• Automated sample-to-report workflow on the Genexus sequencer in less than a day
• Sequencing of up to eight samples (DNA & RNA) per lane on a GX5 Chip in a single run
• Less than 15 minutes hands-on time
• Detection of somatic variants down to 5% allele frequency

The Oncomine Myeloid Assay GX v2 is designed to assist in myeloid cancer research. The DNA panel is comprised of 45 key genes, while the RNA panel includes a broad fusion panel of 30 driver genes, covering the most relevant targets associated with major myeloid disorders, including acute myeloid leukemia (AML), myeloid dysplastic syndrome (MDS), myeloproliferative neoplasms (MPN), chronic myeloid leukemia (CML), chronic myelomonocytic leukemia (CMML), and juvenile myelomonocytic leukemia (JMML).

The multiplex primer design leverages Ion AmpliSeq technology to generate results from multiple samples in a single run. Sequencing results are automatically analyzed by Genexus software using an optimized assay-specific analysis workflow.

The assay kit includes three pools of Ion AmpliSeq oligonucleotide primers (a 2-pool DNA panel and 1-pool RNA panel) and Genexus Strips 1 & 2-AS library reagents, enough to sequence up to 32 samples on the Genexus Integrated Sequencer. One end-to-end sequencing run uses one lane on the GX5 Chip and accommodates up to eight samples. Additional Genexus sequencer reagents and supplies must be ordered separately.