pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ Mach1™-T1^R E. coli and PureLink™ Kit (Invitrogen™)

The pCR™8/GW/TOPO™ TA Cloning Kit delivers simple 5 minute cloning and access to the Gateway™ recombination cloning vector platform. Our pCR™8 TOPO™ TA vector contains a minimal MCS, so you sequence more of your insert and less of the vector. And with our patented att sites, the pCR™8 vector is the simplest way to create an Entry Clone, which facilitates easy access via Gateway™ recombination cloning to a variety of expression vectors without tedious sub-cloning, saving you valuable research time.

The pCR™8/GW/TOPO™ TA Cloning Kit features:
• Fast growing E. coli that shortens cloning time
• Maximum convenience: includes plasmid miniprep kit
• att sites for rapid recombination into any Gateway™ destination vector
• 3'-T overhangs for direct ligation of Taq-amplified PCR products
• Sequencing primer sites within 55 bp of insertion site ensures you sequence more insert, less vector
• Spectinomycin resistance gene for robust selection in E. coli
• EcoR I sites flanking the PCR product insertion site for easy excision of inserts

This kit includes One Shot™ Mach1™-T1^R Chemically Competent E. coli and a PureLink™ Quick Plasmid Miniprep Kit.