POROS™ Ethyl Hydrophobic Interaction Chromatography (HIC) Resin (Thermo Scientific™)

POROS Ethyl Hydrophobic Interaction Chromatography (HIC) Resin is a bioprocess high-performance resin engineered for improved impurity removal during large-scale downstream purification of biomolecules. This unique resin provides high resolution and capacity while maintaining excellent pressure flow characteristics, helping increase productivity.

POROS Ethyl HIC Resin is designed on the well-established cross‐linked poly(styrene-divinylbenzene) POROS base bead with an attached novel hydrophobic ethyl ligand, a resin that is primarily suited for bind/elute purifications.

Features of POROS Ethyl HIC Resin include:
• Superior resolution capability while maintaining excellent pressure-flow properties, achieved by combining the high-flow characteristics of a POROS base bead with a 50 micron particle size. Higher flow rates help improve productivity.
• Unique selectivity due to the novel hydrophobic ligand, allowing for purification of a wide range of biomolecules, including therapeutic proteins, antibody fragments, antibody drug conjugates, and enzymes
• High capacity, reducing the process volume significantly for subsequent steps
• A rigid polymeric bead with covalent surface chemistry for easier handling and packing with superior physical and chemical stability, allowing cleaning with various clean-in-place (CIP) solutions such as sodium hydroxide and enabling a more robust downstream purification process

These features of POROS Ethyl HIC Resin are a major improvement to the performance of traditional HIC resins, setting the standard for hydrophobic interaction chromatography. POROS Ethyl HIC Resin combines superior and consistent performance, exceptional customer support, strong operational excellence, and a strong legacy based on proven technology from a well-established chromatography resin supplier. This resin comes with complete technical and regulatory support. POROS Ethyl HIC Resin is available in various bulk package sizes for large-scale purification, as well as pre-packed columns for initial process development.

Main characteristics

Support matrix: cross-linked poly(styrene-divinylbenzene)
Average particle size: 50 μm
Ligand: novel ethyl
pH range: 1–14
Ionic strength range: 0 to 5 M, all common salts
Mechanical resistance: 100 bar (1,450 psi; 10 MPa)
Shipping solution: 18% (v/v) ethanol