Precision ID Identity and Library Kit Bundle (Applied Biosystems™)

The Applied Biosystems™ Precision ID Identity Panel and Library Kit Bundle is packaged for easy ordering and preparation of amplicon libraries using the Precision ID Identity Panel (Cat. No. A25643) and the Precision ID Library Kit (Cat. No. A26435) for sequencing on Ion Torrent sequencing systems. The Precision ID Identity Panel of multiplexed SNPs provides unique identification of degraded or trace samples with discrimination similar to the STR genotype match probabilities used by forensic analysts (between 1x10^–31 and 6x10^–35).

Simple protocol
• 96 well plate-based workflow enables easy sample management
• No library amplification with qPCR quantification

Optimized digestion reagent
• Cleaner digest allows for more efficient downstream sequencing

Scalable multiplex PCR reactions
The Precision ID Identity Panel is built on Ion AmpliSeq™ chemistry which enables scalable multiplex PCR reactions for all 124 markers in the panel using just 1 ng of starting DNA, with superior coverage uniformity and specificity (acceptable performance has also been observed with sub-nanogram input quantities as low as 180 pg). In addition, the primers contain proprietary modifications that enable removal of primer sequences during library preparation, for efficient target assessment during sequencing. Multiple primer pools can be used to create overlapping amplicons that enable complete coverage of large targets. Learn more about other Precision ID panels >

The included Precision ID Library Kit includes reagents for generating amplicons with Ion AmpliSeq primers and preparing libraries from the resulting amplicons. The kit enables the preparation of barcoded libraries using the Ion Xpress™ Barcode Adapters 1-96 Kit, and the IonCode™ Barcode Adapters 1-384 Kit. Barcoded libraries can be combined and loaded onto a single Ion chip to minimize sequencing run time and cost and allow for accurate sample-to-sample comparisons.

Easy and flexible protocol
The resulting DNA libraries are ready for downstream template preparation for clonal amplification on Ion Sphere™ particles using the automated Ion OneTouch™ 2 System. The intuitive Torrent Suite Software and HID SNP Genotyper plug-in enable you to go from extracted DNA to ancestry-informative genotypes in less than two days*.

* Using the Applied Biosystems™ Precision ID NGS System for Human Identification