5-(and-6)-Carboxy SNARF™-1 (Invitrogen™)

The cell-impermeant pH indicator, carboxy SNARF™-1 dye has a pK_a of ∼7.5, thus is useful for measuring pH changes between pH 7 and pH 8. Carboxy-SNARF™-1 exhibits a significant pH-dependent emission shift from yellow-orange to deep red fluorescence under acidic and basic conditions, respectively. This pH dependence allows the ratio of the fluorescence intensities from the dye at two emission wavelengths - typically 580 nm and 640 nm - to be used for quantitative determinations of pH.

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