AbC™ Total Antibody Compensation Bead Kit (Invitrogen™)

The AbC™ Total Antibody Compensation Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation when using fluorochrome-conjugated mouse, rat, hamster, or rabbit antibodies with any laser in flow cytometry.

• Broadest multispecies reactivity—for use with all isotypes of mouse, rat, hamster, and rabbit antibody conjugates
• Brightest signal—most sensitive antibody-capture beads available
• Maximum laser compatibility—for use with all lasers

Accurate compensation is crucial for effective multicolor analysis in flow cytometry. Often, however, cellular-based compensation controls are not completely effective, as many antigens are not highly expressed, and dimly stained cells can lead to inaccurate compensation settings. The AbC™ Total Antibody Compensation Bead Kit was developed to address this issue.

View a selection guide for all flow cytometry compensation beads.

How it works
Each kit provides polystyrene microspheres that have fluorescent antibody conjugate capture capacity (positive compensation beads) or are inert (negative compensation beads). After mixing with a fluorophore-conjugated mouse, rat, hamster, or rabbit antibody, the two components provide a distinct high-signal positive control with an appropriate negative population that can then be used to set compensation properly regardless of the intensity of the cells in the actual experiment.

Broadest multispecies reactivity
The AbC™ Total Antibody Compensation Bead Kit is ideal for compensation with all isotypes of mouse (IgG1, IgG2a, IgG2b, IgG3, IgM), rat (IgG1, IgG2a, IgG2b, IgG2c, IgM), hamster (Armenian IgG and Syrian IgG), and rabbit (monoclonal and polyclonal) immunoglobulins.

Brightest signal
The AbC™ Total Compensation Bead Kit demonstrates the highest and most consistent reactivity to the different subclasses of immunoglobulin in the industry.

Maximum laser compatibility
All AbC™ compensation bead kits are ideal for use with any excitation source, including the 405 nm laser which has historically provided higher background signals due to auto-fluorescence with other kits on the market.