Annexin V Conjugates for Apoptosis Detection (Invitrogen™)

Achieve quick and reliable detection of early cell apoptosis with Annexin V stand-alone conjugates for apoptosis detection. Annexin V conjugates offer up to 100-fold difference in fluorescence signal intensity between apoptotic and non-apoptotic cells using flow cytometry.

Key Features

Annexin V has a high affinity for phosphatidylserine (PS), which becomes exposed on the outer leaflet of cells undergoing apoptosis. Because of this affinity, fluorescently labeled annexin V reagents are commonly used in apoptosis research.

Annexin V conjugates provide quick and reliable detection methods for studying the externalization of phosphatidylserine, an indicator of intermediate stages of apoptosis. The difference in fluorescence intensity between apoptotic and nonapoptotic cells stained with our fluorescent annexin V conjugates, as measured by flow cytometry, is typically about 100-fold.

In collaboration with Nexins Research BV, we provide the best and brightest annexin V conjugates available, including Alexa Fluor 350, 488, 555, 568, 594, 647, and 680 annexin V conjugates, as well as Annexin V APC, Biotin-X, FITC, Pacific Blue, and PE conjugates. Highly fluorescent annexin V conjugates provide quick and reliable detection methods for studying the externalization of phosphatidylserine, one of the earliest indicators of apoptosis.

The Annexin V Pacific Blue conjugate is violet excitable, making it ideal for instruments with a violet laser and for multicolor experiments that include green- or red-fluorescent dyes.

The benefits of our annexin V conjugates include:
• Conjugated to Invitrogen Alexa Fluor and eFluor dyes for brighter signals
• Conjugates for all available lasers
• Available as stand-alone reagents or easy-to-use kits

Annexin V staining to detect apoptotic cells can only be done on live cells and tissue. If samples are to be fixed post-staining, there are specific conditions required to achieve transient retention of signal. These include use of an alcohol-free, aldehyde-based fixation method, use of buffers containing Ca2+ and avoidance of surfactants/detergents. For your convenience, we also offer a concentrated annexin-binding buffer that facilitates the binding of annexin V to phosphatidylserine in apoptosis assays.

Order Codes

Code Description
A13204 Catalog Number: A13204
Unit Size: 500 µL
Conjugate: Biotin-X
A13201 Catalog Number: A13201
Unit Size: 500 µL
Conjugate: Alexa Fluor 488
Excitation/Emission: 495/519
Flow Cytometer Laser Lines: 488
A13199 Catalog Number: A13199
Unit Size: 500 µL
Conjugate: FITC
Excitation/Emission: 494/518
Flow Cytometer Laser Lines: 488
A13202 Catalog Number: A13202
Unit Size: 500 µL
Conjugate: Alexa Fluor 568
Excitation/Emission: 578/603
Flow Cytometer Laser Lines: 532, 561
A13203 Catalog Number: A13203
Unit Size: 500 µL
Conjugate: Alexa Fluor 594
Excitation/Emission: 590/617
Flow Cytometer Laser Lines: 532
A23202 Catalog Number: A23202
Unit Size: 500 µL
Conjugate: Alexa Fluor 350
Excitation/Emission: 346/442
Flow Cytometer Laser Lines: UV
A23204 Catalog Number: A23204
Unit Size: 500 µL
Conjugate: Alexa Fluor 647
Excitation/Emission: 650/665
Flow Cytometer Laser Lines: 633-637
A35108 Catalog Number: A35108
Unit Size: 500 µL
Conjugate: Alexa Fluor 555
Excitation/Emission: 555/565
Flow Cytometer Laser Lines: 532, 561
A35109 Catalog Number: A35109
Unit Size: 500 µL
Conjugate: Alexa Fluor 680
Excitation/Emission: 679/702
Flow Cytometer Laser Lines: 633-637
A35110 Catalog Number: A35110
Unit Size: 250 µL
Conjugate: APC (Allophycocyanin)
Excitation/Emission: 650/660
Flow Cytometer Laser Lines: 633-637
A35111 Catalog Number: A35111
Unit Size: 250 µL
Conjugate: PE
Excitation/Emission: 565/578
Flow Cytometer Laser Lines: 488, 532, 561
A35122 Catalog Number: A35122
Unit Size: 500 µL
Conjugate: Pacific Blue
Excitation/Emission: 410/455
Flow Cytometer Laser Lines: 405
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