Annexin V Conjugates for Apoptosis Detection (Invitrogen™)

Key Features

Annexin V has a high affinity for phosphatidylserine (PS), which becomes exposed on the outer leaflet of cells undergoing apoptosis. Because of this affinity, fluorescently labeled annexin V reagents are commonly used in apoptosis research.

Annexin V conjugates provide quick and reliable detection methods for studying the externalization of phosphatidylserine, an indicator of intermediate stages of apoptosis. The difference in fluorescence intensity between apoptotic and nonapoptotic cells stained with our fluorescent annexin V conjugates, as measured by flow cytometry, is typically about 100-fold.

In collaboration with Nexins Research BV, we provide the best and brightest annexin V conjugates available, including Alexa Fluor 350, 488, 555, 568, 594, 647, and 680 annexin V conjugates, as well as Annexin V APC, Biotin-X, FITC, Pacific Blue, and PE conjugates. Highly fluorescent annexin V conjugates provide quick and reliable detection methods for studying the externalization of phosphatidylserine, one of the earliest indicators of apoptosis.

The Annexin V Pacific Blue conjugate is violet excitable, making it ideal for instruments with a violet laser and for multicolor experiments that include green- or red-fluorescent dyes.

The benefits of our annexin V conjugates include:
• Conjugated to Invitrogen Alexa Fluor and eFluor dyes for brighter signals
• Conjugates for all available lasers
• Available as stand-alone reagents or easy-to-use kits

Annexin V staining to detect apoptotic cells can only be done on live cells and tissue. If samples are to be fixed post-staining, there are specific conditions required to achieve transient retention of signal. These include use of an alcohol-free, aldehyde-based fixation method, use of buffers containing Ca2+ and avoidance of surfactants/detergents. For your convenience, we also offer a concentrated annexin-binding buffer that facilitates the binding of annexin V to phosphatidylserine in apoptosis assays.

Order Codes

Code	Description
A35122	Catalog Number: A35122 Unit Size: 500 µL Conjugate: Pacific Blue Excitation/Emission: 410/455 Flow Cytometer Laser Lines: 405
A13201	Catalog Number: A13201 Unit Size: 500 µL Conjugate: Alexa Fluor 488 Excitation/Emission: 495/519 Flow Cytometer Laser Lines: 488
A13199	Catalog Number: A13199 Unit Size: 500 µL Conjugate: FITC Excitation/Emission: 494/518 Flow Cytometer Laser Lines: 488
A13202	Catalog Number: A13202 Unit Size: 500 µL Conjugate: Alexa Fluor 568 Excitation/Emission: 578/603 Flow Cytometer Laser Lines: 532, 561
A13203	Catalog Number: A13203 Unit Size: 500 µL Conjugate: Alexa Fluor 594 Excitation/Emission: 590/617 Flow Cytometer Laser Lines: 532
A13204	Catalog Number: A13204 Unit Size: 500 µL Conjugate: Biotin-X
A23202	Catalog Number: A23202 Unit Size: 500 µL Conjugate: Alexa Fluor 350 Excitation/Emission: 346/442 Flow Cytometer Laser Lines: UV
A23204	Catalog Number: A23204 Unit Size: 500 µL Conjugate: Alexa Fluor 647 Excitation/Emission: 650/665 Flow Cytometer Laser Lines: 633-637
A35108	Catalog Number: A35108 Unit Size: 500 µL Conjugate: Alexa Fluor 555 Excitation/Emission: 555/565 Flow Cytometer Laser Lines: 532, 561
A35109	Catalog Number: A35109 Unit Size: 500 µL Conjugate: Alexa Fluor 680 Excitation/Emission: 679/702 Flow Cytometer Laser Lines: 633-637
A35110	Catalog Number: A35110 Unit Size: 250 µL Conjugate: APC (Allophycocyanin) Excitation/Emission: 650/660 Flow Cytometer Laser Lines: 633-637
A35111	Catalog Number: A35111 Unit Size: 250 µL Conjugate: PE Excitation/Emission: 565/578 Flow Cytometer Laser Lines: 488, 532, 561