BacMam Histone H3 Reagent (Invitrogen™)

The BacMam Histone H3 Reagent provides a convenient genetic delivery tool for the expression of GFP-Histone H3 fusion protein in your cell background of interest. The GFP fusion enables sensitive detection of post-translational modifications using LanthaScreen™ TR-FRET Cellular Assay technology.

The BacMam Histone H3 Reagent Lets You:

• Use your cell background of choice with the portability of BacMam
• Conserve precious cell samples with the miniaturizable homogenous assay format
• Identify more relevant inhibitors since the methyl transferases and demethylases are in their natural protein complexes
• Improve data quality with the advantages of TR-FRET

Get More Physiologically Relevant Results
The BacMam Histone H3 allows the investigation of post-translational modifications of histone H3 in your choice of cellular backgrounds, including primary cells. This enables screening for potential inhibitors of histone-modifying enzymes such as kinases, acetylases, deacetylases, methyl transferases, and demethylases in their natural complexes in a physiologically relevant cell type.

BacMam and LanthaScreen™ Convenience Saves Sample and Time
When used with a LanthaScreen™ Tb-anti-Histone H3 antibody against a specific modification, assays can be run in a fully homogenous, addition-only format without any of the washing, lysate transfer, or separation procedures required for traditional methods such as Western blotting and ELISA. By using the same BacMam reagent for GFP-Histone H3, a variety of histone H3 modifications (Ph, Me, Ac) can be monitored just by exchanging the Terbium (Tb) labeled antibody. In addition, the application of the LanthaScreen™ technology includes all of the advantages of TR-FRET detection, including reduced data noise, less interference from fluorescent compounds, and high sensitivity, allowing the use of fewer cells.

For research use only. Not for human or animal therapeutic or diagnostic use.