Calcein, AM, cell-permeant dye (Invitrogen™)

Calcein AM is a cell-permeant dye that can be used to determine cell viability in most eukaryotic cells. In live cells the non-fluorescent calcein AM is converted to green-fluorescent calcein, after acetoxymethyl ester hydrolysis by intracellular esterases. This dye is also available in our special packaging (Cat. No. C-3100) and resuspended in DMSO (Cat. No. C-3099). For a longer wavelength version of this dye check out our new CellTrace calcein red-orange AM (Cat. No. C-34851).

Dissolve the dye using high-quality, anhydrous dimethylsulfoxide (DMSO) up to 1 mg/mL. Calcein AM is susceptible to hydrolysis when exposed to moisture. Aqueous working solutions containing calcein AM should be prepared fresh and and used on same day. Once prepared, use DMSO stock solutions of calcein AM within a short time period for one set of experiments.

Consult user Manual for solubility instructions.

Visualize staining your cell without wasting your reagents, antibodies, or time with our new Stain-iT Cell Staining Simulator.