CellROX™ Deep Red Reagent, for oxidative stress detection (Invitrogen™)

CellROX™ Deep Red Reagent is a novel fluorogenic probe for measuring cellular oxidative stress in both live and fixed cell imaging, with absorption/emission maxima at ∼644/665 nm. The cell-permeant dye is non-fluorescent while in a reduced state, and exhibits bright fluorescence upon oxidation by reactive oxygen species (ROS).

Features of the CellROX™ Deep Red Reagent:
• Optimized probe for detection of oxidative stress in cells
• Simple protocol that can be used with other live cell dyes and GFP
• Compatible with both live cell fluorescence-imaging and formaldehyde-based fixation methods

Substrate Specifics
The CellROX™ Deep Red reagent is a novel cell-permeant dye with absorption/emission maxima of ∼644/665 nm. CellROX™ Deep Red reagent is non-fluorescent while in a reduced state and becomes fluorescent upon oxidation by reactive oxygen species with emission maxima ∼665 nm that is measurable by fluorescent imaging, high content imaging, fluorescent plate readers, or flow cytometry.

Oxidative Stress Detection with CellROX™ Deep Red Reagent
Oxidative stress results from an imbalance between production of reactive oxygen species (ROS) and the ability of cells to scavenge them. ROS play an important role in the progression of several diseases including inflammation, atherosclerosis, aging, and age-related degenerative disorders. CellROX™ Deep Red Reagent can detect the oxidative stress in cells by reacting with ROS to become brightly fluorescent.

Simple, Robust Protocol
The protocol to use this reagent is simple and the bright deep red fluorescence signal is compatible with other live cell dyes and GFP, making it useful in multiplex fluorescence assays to measure a variety of cellular phenomena, including parameters related to cytotoxicity and cell death (Figure 4 below). Furthermore, unlike many other ROS sensors, such as H2DCFDA, the signal from CellROX™ Deep Red is retained after formaldehyde fixation for flexibility with the assay and improved workflows compared to those based on classic dyes for ROS detection.