Dynabeads™ CD14 (Invitrogen™)

Dynabeads™ CD14 are superparamagnetic beads coupled with an anti-human CD14 antibody that enable easy isolation or depletion of human CD14⁺ cells directly from any sample, including whole blood, bone marrow, buffy coat, mononuclear cells (MNC), and tissue digests. Advantages of Dynabeads™ CD14:

• Fast and efficient isolation of CD14⁺ cells from any sample—no columns required
• High purity, yield, and viability of positively isolated CD14⁺ cells for molecular downstream assays
• Fast and efficient depletion of CD14⁺ cells from any sample

About Dynabeads™ CD14
Dynabeads™ CD14 are uniform, superparamagnetic beads (4.5 μm diameter) coated with a primary monoclonal antibody specific for the CD14 membrane antigen mainly expressed mainly on human monocytes. Because of the bead size, Dynabeads™ CD14 can easily and efficiently isolate or deplete cells from viscous samples such as whole blood and bone marrow in about 30 minutes. Dynabeads™ CD14 will also afford excellent recovery of high-purity, viable cells for use in downstream molecular studies; for example, those in which cells are to be lysed while still attached to beads, and nucleic acids or proteins further purified. Note that intact cells will not be released from these beads, so if you want to isolate CD14⁺ cells for cell-based applications, or you need to check your samples with flow cytometry, check out our total human monocyte product offerings.

Magnetic bead–based separation offers easy handling
Dynabeads™ CD14 is added to the sample under continuous mixing to optimize the binding of the Dynabeads™ to the target cells. By placing the sample on a magnet, it separates the bead-bound target cells from the rest of the sample in just 1–2 minutes. For depletion, remove the supernatant to a new tube for further studies and discard the bead-bound cells. For positive isolation for molecular studies, remove the supernatant and wash the bead-bound cells 2–3 times in buffer to get optimal purity. The cells can be lysed while still attached to the beads and the supernatant transferred to a new tube for downstream molecular analysis. Starting samples can be whole blood, buffy coat, bone marrow, PBMC, or tissue digests.

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