Dynabeads™ M-270 Epoxy (Invitrogen™)

Dynabeads™ M-270 Epoxy beads are 2.8 μm superparamagnetic beads containing surface epoxy groups. The beads covalently bind primary amino and sulfhydryl groups in proteins and peptides, making them ideal for coupling antibodies, peptides, intact proteins, and functional enzymes. These hydrophylic, neutral pH beads exhibit extremely low nonspecific binding of proteins and dyes, which reduces the need for blocking agents. Also, Dynabeads™ M-270 Epoxy Beads do not contain Tween™ detergent, and so are ideal for use in mass spectrometry (MS) analysis.

• Ideal for immunoprecipitation (IP) of proteins and protein complexes
• Gentle, yet rapid magnetic separation and short incubation times allow identification of transient and labile complexes
• Covalent antibody-coupling to the Dynabeads™ avoids co-elution of the antibody with the target protein

Adaptable for various applications
Fast surface binding kinetics and rapid magnetic separation characteristics make Dynabeads™ M-270 Epoxy beads the ideal choice for applications such as immunoprecipitation/purification of proteins and protein complexes, coupling functional enzymes to the bead surface for downstream assays, and identifying protein binding partners. Captured proteins and protein complexes are easily separated, washed, and eluted using a DynaMag™ magnet, and the magnetic separation properties of the Dynabeads™ .

Coupling procedure
Covalent coupling is performed overnight by incubating the desired ligand (commonly antibodies, peptides, or proteins) with Dynabeads™ M-270 Epoxy beads. The binding capacity varies depending on the ligand used, but is typically 5–10 μg IgG per milligram of beads. Coupling occurs at neutral pH and high salt concentration. The coupling reaction can be performed over a broad range of temperatures to match the stability characteristics of your ligand.

Upon completion of the ligand coupling step, the reactive moieties on the Dynabeads™ M-270 Epoxy beads are deactivated to prevent further coupling. Once the coupling reaction is completed, these nonporous, hydrophilic, pH neutral beads exhibit extremely low nonspecific binding.

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