eBioscience™ Annexin V Apoptosis Detection Kits (Invitrogen™)

Easily distinguish cells undergoing early and late apoptosis with eBioscience and Annexin V Apoptosis Detection kits for flow cytometry analysis. These kits utilize viability dye-conjugated annexin V to label phosphatidylserine (PS), an early marker of apoptosis, on the extracellular membrane. In late-stage apoptosis, as cell integrity is lost, both annexin V and the viability dye traverse and bind PS on the interior of the plasma membrane.

Key Features

Annexins are a family of calcium-dependent phospholipid-binding proteins that preferentially bind phosphatidylserine (PS). Under normal physiologic conditions, PS is predominantly located in the inner leaflet of the plasma membrane. Upon initiation of apoptosis, PS loses its asymmetric distribution across the phospholipid bilayer and is translocated to the extracellular membrane leaflet marking cells as targets of phagocytosis. Once on the outer surface of the membrane, PS can be detected by fluorescently labeled annexin V in a calcium-dependent manner.

In early-stage apoptosis, the plasma membrane excludes viability dyes such as propidium iodide (PI), 7-AAD, or fixable viability dyes such as eFluor 450. These cells will stain with annexin V but not a viability dye, thus distinguishing cells in early apoptosis. However, in late-stage apoptosis, the cell membrane loses integrity, thereby allowing annexin V to also access PS in the interior of the cell. A viability dye can be used to resolve these late-stage apoptotic and necrotic cells (annexin V, viability dye-positive) from the early-stage apoptotic cells (annexin V positive, viability dye-negative).

Current viability stains available with the eBioscience Annexin V Apoptosis Detection kits include FITC, eFluor 450, allophycocyanin (APC), phycoerythrin (PE), PE-cyanine7, and biotin.

Order Codes

Code	Description
88-8103-72	Catalog Number: 88-8103-72 Unit Size: 50 tests Conjugate: PE/Cy7 Quantity: 50 Tests
88-8005-74	Catalog Number: 88-8005-74 Unit Size: 200 tests Conjugate: FITC, Propidium Iodide Excitation/Emission: 488, 535/520, 617 Quantity: 200 Tests
88-8006-72	Catalog Number: 88-8006-72 Unit Size: 50 tests Conjugate: eFluor 450, 7-AAD Excitation/Emission: 405, 546/450, 647 Quantity: 50 Tests
88-8006-74	Catalog Number: 88-8006-74 Unit Size: 200 tests Conjugate: eFluor 450, 7-AAD Excitation/Emission: 405, 546/450, 647 Quantity: 200 Tests
88-8007-74	Catalog Number: 88-8007-74 Unit Size: 200 tests Conjugate: APC, Propidium Iodide Excitation/Emission: 650, 535/660, 617 Quantity: 200 Tests
88-8008-72	Catalog Number: 88-8008-72 Unit Size: 50 tests Conjugate: PCP-eFluor 710 Quantity: 50 Tests
88-8008-74	Catalog Number: 88-8008-74 Unit Size: 200 tests Conjugate: PCP-eFluor 710 Quantity: 200 Test
BMS500FI-100	Catalog Number: BMS500FI-100 Unit Size: 100 tests Conjugate: FITC, Propidium Iodide Excitation/Emission: 488, 535/520, 617 Quantity: 100 Tests
BMS500FI-20	Catalog Number: BMS500FI-20 Unit Size: 20 tests Conjugate: FITC, Propidium Iodide Excitation/Emission: 488, 535/520, 617 Quantity: 20 Tests
BMS500FI-300	Catalog Number: BMS500FI-300 Unit Size: 300 tests Conjugate: FITC, Propidium Iodide Excitation/Emission: 488, 535/520, 617 Quantity: 300 Tests
88-8102-72	Catalog Number: 88-8102-72 Unit Size: 50 tests Conjugate: PE, 7-AAD Quantity: 50 Tests
88-8102-74	Catalog Number: 88-8102-74 Unit Size: 200 tests Conjugate: PE, 7-AAD Quantity: 200 Tests
88-8103-74	Catalog Number: 88-8103-74 Unit Size: 200 tests Conjugate: PE/Cy7 Quantity: 200 Tests
BMS500BT-100	Catalog Number: BMS500BT-100 Unit Size: 100 tests Conjugate: Biotin, Propidium Iodide Excitation/Emission: NA, 535/NA, 617 Quantity: 100 Tests
BMS500BT-20	Catalog Number: BMS500BT-20 Unit Size: 20 tests Conjugate: Biotin, Propidium Iodide Excitation/Emission: NA, 535/NA, 617 Quantity: 20 Tests
BMS500BT-300	Catalog Number: BMS500BT-300 Unit Size: 300 tests Conjugate: Biotin, Propidium Iodide Excitation/Emission: NA, 535/NA, 617 Quantity: 300 Tests
88-8005-72	Catalog Number: 88-8005-72 Unit Size: 50 tests Conjugate: FITC, Propidium Iodide Excitation/Emission: 488, 535/520, 617 Quantity: 50 Tests
88-8007-72	Catalog Number: 88-8007-72 Unit Size: 50 tests Conjugate: APC, Propidium Iodide Excitation/Emission: 650, 535/660, 617 Quantity: 50 Tests