eBioscience™ CyTRAK Orange™ (Invitrogen™)

CyTRAK Orange™ is an anthraquinone dye with high affinity for double-stranded DNA. It is a membrane-permeable dye that can label live or fixed/dead cells. In flow cytometry, it can be used to distinguish nucleated and non-nucleated cells. In fluorescent microscopy, it can be used to identify and discriminate the nucleus and cytoplasm without the need for a second dye due to its high intensity staining of the nucleus and low intensity staining of the cytoplasm.

CyTRAK Orange™ is optimally excited from 488 to 550 nm and has a peak emission of 610 nm. It can be excited with the blue laser (488 nm). For a filter configuration, CyTRAK Orange™ is best detected with a 610/20 band pass filter or equivalent. Please make sure that your instrument is capable of detecting this dye.

Reported Application
Microscopy, Flow Cytometric Analysis, Cell Labeling, Immunocytochemistry