Fluorescein Phalloidin (Invitrogen™)

Fluorescein phalloidin is a high-affinity F-actin probe conjugated to the green fluorescent dye, fluorescein (FITC).

• Selectively stains F-actin
• Excitation/Emission: 496/516 nm
• Superior to antibody staining
• Optimal for fixed and permeabilized samples

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides 'death cap' mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while fluorescein provides green fluorescence. Demonstrating very little nonspecific staining, fluorescein phalloidin allows high-contrast discrimination of actin staining.

Use in Multiple Applications
Fluorescein phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Fluorescein phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot nanocrystals, and Alexa Fluor conjugates including secondary antibodies. For research use only.

Not intended for human or animal therapeutic or diagnostic use.

Related Links
• Read about labeling F-Actin with phallotoxins.
• Create a Cellular Masterpiece with Alexa Fluor Phalloidin using the Cell Paint Tool.

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