HCS LipidTOX™ Phospholipidosis and Steatosis Detection Kit, for high-content screening, for cellular imaging (Invitrogen™)

HCS LipidTOX™ Phospholipidosis and Steatosis Detection Kit offers a complete set of reagents for performing image-based high-content screening (HCS) assays to characterize phospolipidosis and steatosis, toxic side effects on lipid metabolism that can be triggered by drugs or other compounds. The kit supplies LipidTOX™ Red phospholipid stain and LipidTOX™ Green neutral lipid stain, which can be used sequentially for the analysis of phospholipidosis and steatosis, respectively, or can be used in isolation for single-parameter analysis. Both fluorescent probes can be easily detected with fluorescence microscopes and can be quantified with stand-alone image analysis software or the built-in image analysis software of most HCS readers. In addition to the phospholipid and neutral lipid stains, this kit supplies the nuclear stain Hoechst 33342, propranolol and cyclosporin A (control compounds), and DMSO. Sufficient reagents are supplied for 2, 96-well plates (H34157) or 10, 96-well plates (H34158).