Image-iT™ Fixation/Permeabilization Kit (Invitrogen™)

The Image-iT Fixation/Permeabilization Kit is a convenient-to-use fixation/permeabilization kit that contains all the necessary reagents to prepare cells for antibody staining and imaging. The high-quality components help preserve cell morphology and reduce background staining and are ideal for use with antibody-based detection methods, including immunocytochemistry (ICC), immunofluorescence (IF), and immunohistochemistry (IHC). An easy-to-follow protocol is also provided. Note: the 4% formaldehyde, methanol-free fixative solution included in this kit is also available for separate purchase (see Cat. No. R37814).

Features of the Image-iT Fixation/Permeabilization Kit include:
• A complete fixation/permeabilization kit with ready-to-use components and room temperature storage
• Optimized for secondary antibody labeling, fixed-cell dye staining, and GFP applications
• Reduced background staining

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The Image-iT Fixation/Permeabilization Kit contains:
• Fixative: high purity 4% formaldehyde (methanol-free) in PBS, pH 7.3
• Permeabilization solution: 0.5% Triton X-100
• Blocking buffer: 3% BSA, fraction V, de-lipidated, New Zealand source, in DPBS
• Wash solution: DPBS, no calcium, no magnesium