Oregon Green™ 488 Phalloidin (Invitrogen™)

Oregon Green 448 phalloidin is a high-affinity F-actin probe conjugated to green fluorescent Oregon Green 488 dye.

• Selectively stains F-actin
• Outstanding fluorescence performance
• Excitation/Emission: 496/520 nm
• Superior to antibody staining
• Optimal for fixed and permeabilized samples
• pH insensitive under physiological conditions

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides 'death cap' mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while Oregon Green 488 provides green fluorescence with brightness superior to fluorescein. Oregon Green 488 is a fluorinated analog of fluorescein with greater photostability and a lower pKa, making its fluorescence essentially pH insensitive in the physiological pH range. Demonstrating very little nonspecific staining, Oregon Green 488 phalloidin allows high-contrast discrimination of actin staining.

Use in Multiple Applications
Oregon Green 448 phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Oregon Green 448 phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot nanocrystals, and Alexa Fluor conjugates including secondary antibodies.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links
• Read about labeling F-Actin with phallotoxins.
• Create a Cellular Masterpiece with Alexa Fluor Phalloidin using the Cell Paint Tool.

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