SYTOX AADvanced™ Ready Flow™ Reagent (Invitrogen™)

SYTOX AADvanced Ready Flow Reagent is a high-affinity nucleic acid stain for the detection of dead cells in flow cytometry. The dye has excitation/emission maxima of 546/647 nm.

• Stable at room temperature
• Convenient, ready-to-use format—no need to dilute, weigh, or pipette
• Rapid uptake and low coefficient of variation (CV)

SYTOX AADvanced Ready Flow Reagent is a ready-to-use, direct-to-sample dead cell stain that emits red fluorescence when bound to DNA. Add 2 drops form the dropper bottle directly into 1 x 10⁶ cells, incubate, and analyze.

The SYTOX AADvanced Ready Flow Reagent dye is spectrally similar to 7-AAD, but with rapid uptake kinetics and relatively low CVs . It penetrates cells more efficiently than 7-AAD, providing better separation of live and dead cells.