CMRL Medium, no glutamine (Gibco™)

CMRL medium was originally developed by Connaught Medical Research Laboratories for the growth of Earle's 'L'cells under serum-free conditions. CMRL medium is also especially useful for cloning monkey kidney cells and for growth of many other mammalian cell lines when supplemented with horse or calf serum.

The complete formulation is available.

Gibco™ CMRL medium is a modified version of Medium 199. CMRL medium is particularly rich in nucleosides and vitamins

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law.

CMRL medium contains no proteins or growth factors. Therefore, CMRL medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). CMRL medium uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.