Express Five™ SFM (Gibco™)

Express Five™ SFM is a protein-free, serum-free medium specifically developed to support the long-term suspension growth of High Five™ (BTI-TN-5B1-4) cells in suspension for use in the Baculovirus Expression Vector System (BEVS). This medium requires the addition of 18 mM L-glutamine before use. Gibco™ Express Five™ SFM features:

• Superior long-term suspension growth of High Five™ cells
• Protein-free, serum-free formulation
• Optimized for recombinant protein production
• Scalable in the CelliGen™ bioreactor and WAVE Bioreactor™ System

Superior growth of High Five™ cells
Gibco™ Express Five™ SFM provides peak cell densities of up to 6 × 10⁶ viable cells per mL in shaker cultures. Cells adapted to other commercially available serum-free media can be subcultured directly into Gibco™ Express Five™ SFM, usually without any further adaptation (see figure). Cells usually require adaptation from serum-containing formulations.

Protein-free, serum-free formulation
Gibco™ Express Five™ SFM is a protein-free and serum-free formulation, allowing for easier purification of your protein of interest.

Optimized for recombinant protein expression
Gibco™ Express Five™ SFM is optimized for high protein production using the Baculovirus Expression Vector System (BEVS). Using BEVS, we routinely achieve rβ-galactosidase protein expression of up to 1.7 U/cell, a significant improvement over other media (see figure)

Scalable in the CelliGen™ bioreactor and WAVE Bioreactor™ System
Protein production using Gibco™ Express Five™ SFM can be scaled up in bioreactors such as the 5 L CelliGen™ bioreactor and the WAVE Bioreactor™ System. The appropriate impeller or rocking speed and seeding density should be optimized for each system. Depending on the bioreactor design, it may be necessary to supplement Gibco™ Express Five™ SFM with additional Pluronic™ F-68 to avoid sheer stress in the culture.

cGMP manufacturing and quality system
Gibco™ Express Five™ SFM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.