GeneArt™ Cryopreservation Kit for Algae (Invitrogen™)

The GeneArt™ Cryopreservation Kit for Algae is used to preserve algal strains and clones for storage at -80°C for years, thus eliminating liquid nitrogen storage and continuous cultures as a way to maintain your clones. Cryopreservation is the optimal choice for preservation and long-term storage of algae, since it minimizes genetic drift, facilitates strain and clone exchange between labs, and helps reduce maintenance labor and costs. Simply grow your cells in the presence of Cryopreservation Reagent A for 2–5 days, harvest, resuspend in Cryopreservation Reagent B, and freeze a small aliquot in one of two recommended freezing containers* at -80°C. The GeneArt™ Cryopreservation Kit for Algae has been used to preserve Chlamydomonas and Chlorella strains with 100% resuscitation after thawing.

• Preserve Chlamydomonas and Chlorella strains and clones for -80°C storage
• Eliminate liquid nitrogen storage
• Minimize contamination and genetic drift that typically occur in continuous cultures
• Ship algal clones on dry ice for exchange between labs

To date, most available methods for algae cell preservation have required liquid nitrogen storage, but this method is inconvenient and expensive. Alternatively, researchers have relied on continuous cultures to maintain algal clones, but this often leads to contamination and genetic drift and is not amenable to shipping. The GeneArt™ Cryopreservation Kit for Algae provides a way to freeze algae cells at -80°C for long-term storage or shipment on dry ice with minimized loss in viability.

*We have used the following freezing containers with this product:
• Thermo Scientific™ Mr. Frosty™ Freezing Container
• Biocision™ CoolCell™ FTS30 Cell Freezing Container