GlutaMAX™ Supplement (Gibco™)

Gibco GlutaMAX Supplement is an alternative to L-glutamine, with increased stability that improves cell health. GlutaMAX Supplement is suitable for both adherent and suspension culture of mammalian cells, with no adaptation required. GlutaMAX Supplement is offered as a 200 mM L-alanyl-L-glutamine dipeptide in 0.85% NaCl. GlutaMAX Supplement is also included in a variety of media formulations. Compared to L-glutamine, GlutaMAX Supplement:

• Minimizes toxic ammonia build-up
• Improves cell viability and growth
• Remains stable across a wide range of temperatures

Minimizes toxic ammonia build-up
Unlike L-glutamine, GlutaMAX Supplement does not spontaneously break down to form ammonia. Instead, cells cleave the dipeptide bond to release L-glutamine as needed. This system prevents waste build-up and maintains a fresh supply of L-glutamine during long-term culture.

Improves cell viability and growth
Cultures with lower levels of ammonia show improved cell viability. In addition, culture conditions are more controlled due to the consistent concentration of GlutaMAX Supplement that is not possible with L-glutamine.

Remains stable across a wide range of temperatures
GlutaMAX Supplement is room-temperature stable and ready to use. GlutaMAX Supplement retains full functionality for 24 months at room temperature, 2 to 8°C, or -5 to -30°C for convenient storage and handling. GlutaMAX Supplement is even stable at 37°C for a week or more.