HepExtend™ Supplement (50X) (Gibco™)

HepExtend™ Supplement (50X) has been optimized for use with cryopreserved primary hepatocytes to improve cell viability, function, and number of days in culture. This enables researchers to perform metabolic and toxicity experiments not achievable using standard culture conditions. Simply add HepExtend™ Supplement to standard William’s E Hepatocyte Maintenance Media and use with your current hepatocyte culturing methods and instrumentation. The supplement does not contain any small molecules or fetal bovine serum, components that are known to interfere with primary hepatocyte function.

• Enabling—designed to provide maximum cell viability and cell health when culturing primary plateable hepatocytes
• Extended cell survival—days in culture can be extended to >10 days while maintaining normal cell morphology and bile canaliculi
• Consistent—manufactured in a cGMP-compliant facility using the highest quality materials

Maintain P450 activity
HepExtend™ Supplement can be used with your standard hepatocyte culture media to help facilitate metabolically competent hepatocytes for at least 10 days for use in downstream ADME/Tox applications not achievable with standard 5-day culture systems.

Enhanced gene expression
HepExtend™ supplementation increases the basal gene expression of many key genes involved in drug metabolism and transport.

Retain inducibility
Nuclear receptor activation and gene inducibility is retained in cultures treated with HepExtend™ supplemented media.