Human Keratinocyte Growth Supplement (HKGS) (Gibco™)

Human Keratinocyte Growth Supplement (HKGS) is a sterile, concentrated (100X) solution intended for use with EpiLife medium or Medium 154 to culture human epidermal keratinocytes.

HKGS is an ionically-balanced supplement containing bovine pituitary extract (BPE), recombinant human insulin-like growth factor-I, hydrocortisone, bovine transferrin, and human epidermal growth factor. Each 5-mL bottle of HKGS contains all of the growth factors, hormones, and tissue extracts necessary for the culture of human epidermal keratinocytes and is the correct amount of supplement for a 500-mL bottle of medium.

When a 500-mL bottle of medium is supplemented with HKGS, the final concentrations of the components in the supplemented medium are:
• Bovine pituitary extract (BPE): 0.2% v/v
• Recombinant human insulin-like growth factor-I: 0.01 μg/mL
• Hydrocortisone: 0.18 μg/mL
• Bovine transferrin: 5 μg/mL
• Human epidermal growth factor: 0.2 ng/mL