Trypsin-EDTA (0.25%), phenol red (Gibco™)

This liquid formulation of trypsin contains EDTA and phenol red. Gibco Trypsin-EDTA is made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength, trypsin is widely used for cell dissociation, routine cell culture passaging, and primary tissue dissociation. The trypsin concentration required for dissociation varies with cell type and experimental requirements. See the complete range of Gibco trypsin solutions and recommended dissociation conditions.

This trypsin solution is modified as follows:
With
• EDTA
• Phenol Red

The complete formulation is available.

We offer a variety of trypsin formulations, including animal origin-free TrypLE reagents.

Quality testing
Gibco Trypsin solutions are tested for pH, osmolality, sterility, and performance. In addition, prior to manufacturing, the raw materials are verified for e-beam irradiation and tested for endotoxin, PPV, PCV 1/2, mycoplasma, bacterial, fungal, and viral contamination, as well as multiple activity assays, ash analysis, and moisture analysis.

Documented traceability
We can provide detailed documentation to meet your regulatory needs. Gibco Trypsin information available includes lot traceability, animal origin certificates, lot analyses, irradiation certificates, a viral inactivation summary, and supply chain transparency.

cGMP manufacturing and quality system
For supply chain continuity, we manufacture Gibco Trypsin-EDTA (0.25%), phenol red at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements and are certified to the ISO 13485 standard.