ReadyProbes™ 2.5% Normal Horse Serum (1X) (Invitrogen™)

ReadyProbes 2.5% Normal Horse Serum is a ready-to-use non-specific blocker for use in immunohistochemistry (IHC), immunocytochemistry (ICC), and immunofluorescence (IF) experiments, usually when a horse or donkey secondary antibody is used. Incubating cells/tissue with diluted serum from the same or similar species as the host of the secondary antibody typically reduces non-specific binding. Simply cover the tissue section or cells with a few drops of ReadyProbes 2.5% Normal Horse Serum and incubate for 30–60 minutes at room temperature. Ready Probes 2.5% Normal Horse Serum can also be used to dilute primary and secondary antibodies.

Ready Probes 2.5% Normal Horse Serum is provided as a ready-to-use reagent with no further dilution or preparation required. The serum is collected from healthy adult animals and the heat-treated, centrifuged, and filtered. It is then checked for cross reactivity using an appropriate immunoassay. The serum is diluted to 2.5% (v/v) in buffer, with 0.08% sodium azide added as a preservative.