Lauryldimethylamine-N-oxide (LDAO), LC/MS grade (Thermo Scientific™)

Lauryldimethylamine-N-oxide (LDAO) is a zwitterionic surfactant that possesses unique properties for solubilization and stabilization of biomolecules, particularly membrane proteins. LDAO shows particular utility in native mass spectrometry (MS) to study the intact structure and non-covalent interactions of proteins and protein complexes. Incorporation of LDAO into sample preparation can help maintain the integrity of non-covalent interactions, such as protein-protein or protein-ligand interactions, during the transfer from solution to gas phase.

Features of LDAO include:
• Zwitterionic detergent
• Mildness and biocompatibility
• Enhances the stability of solubilized proteins, including membrane proteins
• High-purity compound with low UV absorptivity
• Native mass spec compatible

Applications
LDAO finds applications in various experimental techniques in addition to native MS, including protein purification, crystallization, and structural biology studies. It is compatible with a wide range of biomolecules and can be employed for both soluble and membrane protein research.

Usage
It is important to optimize the concentration of LDAO to ensure that it provides effective solubilization and stabilization of the biomolecules while avoiding excessive detergent interference in the mass spectrometry analysis. Additionally, the specific conditions and parameters of the native MS experiment, such as the choice of ionization source and mass spectrometer parameters, should be carefully considered and optimized for each specific application.

Properties
• CMC: (H₂O) ~1–2 mM (0.023 %)
• Aggregation number: (H₂O) ~76
• Purity: ≥99% by HPLC analysis (all amine oxides) with <2% other DAOs
• pH: 6–8.5 (1% solution in water)
• Solubility: ≥30% (in water at 20°C)
• Fluorescence: <10% (0.1 solution in water at 345 nm)
• Absorbance of a 1% solution in water: 260 nm: <0.08, 280 nm: <0.06, 340 nm: <0.02