Dionex™ AminoPac™ PA10 IC Columns (Thermo Scientific™)

Key Features

The Dionex AAA-Direct system eliminates the need for any form of derivatization, and is therefore compatible with on-line monitoring of cell cultures and fermentation broths. Vitamins, amino sugars, and carbohydrates can be detected simultaneously with amino acids. Additional capabilities include separation of a wide range of sugars, phosphorylated amino acids, and common oxidation products of sulfur-containing amino acids, such as cysteic acid, methionine sulfone, and methionine sulfoxide.

• Carbohydrates and amino acids separated and detected simultaneously
• Mid-femtomole to low-picomole detection limits
• Compatible with all commonly used hydrolysis procedures
• Can be configured for on-line analysis

Thermo Scientific offers two approaches to amino acid analysis: anion-exchange separation by the Dionex AAA-Direct system, in which amino acids are detected directly without the need for derivatization; and anion-exchange separation with postcolumn derivatization.

• Dionex AminoPac PA10 columns separate free amino acids without the need for derivatization.
• Dionex AminoPac PA10 columns separate free amino acids with derivatization.
• Both columns are compatible with solvent, high pH, and high temperatures.
• Both columns facilitate easy scale-up.

Dionex AminoPac columns are used for high-resolution separations of free amino acids as well as for separation and detection of a wide range of sugars, phosphorylated amino acids, and common oxidation products of sulfur-containing amino acids.

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