Axiom™ Maize Genotyping Array (Applied Biosystems™)

Axiom Maize Genotyping Array was designed by the AgroClustER Synbreed (funded by the German Ministry of Education and Research, BMBF) through a collaboration led by Professor Chris-Carolin Schön, Dr. Eva Bauer, and Sandra Unterseer (Technische Universität München, Germany), and Dr. Georg Haberer and Michael Seidel (Helmholtz Zentrum Mnchen, Germany) through Affymetrix Expert Design Program.* The array interrogates 616,201 carefully selected polymorphic variants to represent the global diversity in the maize genome.

The array overcomes specific challenges caused by a high content of repetitive elements, high genomic and structural diversity, and low linkage disequilibrium (LD) in different cultivars. The SNPs on the array were first discovered through resequencing in a panel of 30 inbred lines that represented global diversity. The array was validated by using a diverse set of European, US, and tropical/semi-tropical lines from various Dent and Flint gene pools.

Features of Axiom Maize Genotyping Array:
• Comprehensive content – 616,201 variants including 609,442 SNPs and 6,759 small insertion/deletion variants

Applications of Axiom Maize Genotyping Array:
• Complex trait research
   - Genetic diversity analysis
   - Association mapping
   - Genome-wide analysis and selection
   - Haplotype structure in maize varieties
   - LD analysis
   - QTL mapping
• Molecular breeding
   - Development of new inbred lines
   - Genomic selection

*Expert Design Arrays are designed by key opinion leaders in the plant and animal genomics community and made available to the public for the purpose of advancing research and breeding programs. These arrays contain genetic markers and annotations that are provided by the original customer who designed the array and are therefore not updated by Affymetrix in the same manner as other catalog products. Through the program, Affymetrix continually provides agrigenomics researchers access to genotyping arrays that have been utilized by their peers in the research community.

Required Products
Axiom Analysis Suite
GeneChip Command Console Software
GeneTitan Multi-Channel Instrument