TaqMan™ Array Rodent MicroRNA A+B Cards Set v3.0 (Applied Biosystems™)

The TaqMan™ Array Rodent MicroRNA A+B Cards Set v3.0 is a two card set containing a total of 384 TaqMan™ MicroRNA Assays per card. The set enables accurate quantitation of 641 and 373 unique microRNAs for mouse and rat respectively in as little as 5 hours. Included on each array is three TaqMan™ MicroRNA Assay endogenous controls for each species to aid in data normalization and one TaqMan™ MicroRNA Assay not related to rodent as a negative control. Detail on the included assays is available in the Target List file. Purchase of the Megaplex™ RT Primers, Rodent Pool Set v3.0 is required to run this array set. An additional preamplification step is enabled by Megaplex™ PreAmp Primers, Rodent Pool Set v3.0 for situations where sensitivity is of the utmost importance and/or sample is limiting.

TaqMan™ Array MicroRNA Cards provide all the advantages of TaqMan™ MicroRNA Assays in a convenient, pre-configured microfluidic card, minimizing the experimental variability and effort required to run 384 TaqMan™ MicroRNA Assays in parallel.

Megaplex™ RT Primers are highly multiplexed RT primers designed to convert up to 381 miRNAs to cDNA prior to real-time analysis, and are required to run the TaqMan™ Array MicroRNA Card. For greater sensitivity, or when working with limited sample, Megaplex™ PreAmp Primers provide an optional preamplification step prior to real-time analysis. TaqMan™ Array MicroRNA Cards and Megaplex™ Primer Pools enable a comprehensive expression profile consistent with miRBase v21 to be created in as little as 5 hours, providing the ideal miRNA profiling solution. Key product features:

Highly specific—quantitate only the biologically active mature miRNAs

• Highly streamlined workflow reduces the number of RT (reverse transcription) reactions per sample
• Simplify sample handling and increase sample throughput
• Coverage for human, mouse and rat species
• An ideal miRNA profiling solution

Simplify Sample Handling and Increase Sample Throughput
Following reverse transcription of miRNA targets using Megaplex™ RT Primers and optional preamplification using Megaplex™ PreAmp Primers, TaqMan™ Universal PCR Master Mix II is simply combined with each reaction and pipetted into each sample loading port in the TaqMan™ Array Card, simplifying sample handling and increasing sample throughput.

Provides Comprehensive Coverage of Sanger miRBase
Megaplex™ RT Pools and PreAmp Primers are available for human and rodent species, and consist of pre-selected and matched content across a series of primer pools and TaqMan™ Array Cards. The content is derived from miRBase v14 and v15 for human and rodent species respectively, providing close to full coverage of Sanger with the most up-to-date TaqMan™ MicroRNA Assays.