3' RACE System for Rapid Amplification of cDNA Ends (Invitrogen™)

3´ RACE System is suitable for rapid amplification of cDNA ends (RACE) (1-3) and anchored PCR between a defined point within mRNA and the 3´ poly(A) end (Figure 1). The system is useful for the amplification of rare messages for which little sequence information is available, and for capturing the 3´ end information of mRNA. The 3´ RACE System:

• Can be used with the 5´ RACE System to clone full-length sequences
• Uses the advanced capabilities of SuperScript™ II RT for greater first-strand cDNA yields
• Includes Control RNA and primers to monitor the performance of the system
• Provides detailed protocols in the manual
• Converts up to 1 μg of total RNA preparation or 100 ng of control RNA into cDNA in one reaction