5' RACE System for Rapid Amplification of cDNA Ends, version 2.0 (Invitrogen™)

The 5´ RACE System for Rapid Amplification of cDNA Ends, Version 2.0, is suitable for rapid amplification of cDNA ends (RACE) between a defined point in the mRNA and the 5´ end. The 5' RACE System provides a set of prequalified reagents intended for synthesis of first-strand cDNA, purification of first-strand products, homopolymeric tailing, and preparation of target cDNA for subsequent amplification by PCR. Control RNA, DNA, and primers are provided for monitoring system performance. The system is useful for the amplification of rare messages for which little sequence information may be available, and for capturing the 5´ end information of mRNA. The 5´ RACE system:

• Uses the advanced capabilities of SuperScript™ II RNase H- RT for greater first-strand cDNA yields and increased cDNA lengths
• Prepares specific cDNA from up to 1 μg of total or poly(A)+ RNA in one reaction
• Offers improved first-strand cDNA purification procedure that yields cDNA suitable for tailing
• Uses recombinant TdT with optimized reaction conditions in a PCR-compatible buffer to provide an efficient primer annealing site for PCR