hPSC Scorecard Assay Service (Thermo Scientific™)

hPSC Scorecard Assay Service is a fast and cost-effective alternative to teratoma formation for verification of trilineage differentiation potential in human pluripotent stem cell lines. This service leverages the TaqMan hPSC Scorecard Panel, consisting of 94 predefined TaqMan Gene Expression assays (including endogenous controls) dried-down in the wells. The panel consists of genes associated with the following developmental states: pluripotency, ectoderm, endoderm, and mesoderm.

The hPSC Scorecard Assay Service offers:

• Fast data delivery – turnaround time of four weeks or less
• Convenient sample prep – just send a frozen cell pellet
• Quantitative results – compare scores to reference set of well-characterized pluripotent stem cell lines
• Peer endorsement – cited >240 times in scientific literature
• Exceptional support – a dedicated project manager for frequent project communication

How the hPSC Scorecard Assay Service works:

• You send a pellet with two million cryopreserved cells to our laboratory for processing.
• We isolate high-quality mRNA.
• We generate cDNA using the High-capacity cDNA Reverse Transcription Kit.
• We load cDNA samples into the hPSC Scorecard Panel plate.
• We process the plates on the QuantStudio 12K Flex Real-Time PCR System.
• We analyze your data using hPSC Scorecard Analysis Software.

What you get

You will receive a comprehensive report that includes a box plot with your sample scores and a gene expression heat map that visually represents the expression level of each gene in the panel relative to that of the undifferentiated reference set.