CIAP (Calf Intestinal Alkaline Phosphatase), 1 U/μL (Invitrogen™)

Calf Intestinal Alkaline Phosphatase (CIAP) is a phosphomonoesterase that removes 3´ and 5´ phosphates from DNA and RNA.

Concentration: 1 unit/μL

Applications:
Dephosphorylation of 5´-phosphorylated termini of vector DNA to prevent self-ligation (1). Dephosphorylation of 5´ termini of nucleic acids prior to forward reaction with kinase.

Source: Purified from calf intestinal mucosa.

Performance and Quality Testing:
Endodeoxyribonuclease, 3´ exodeoxyribonuclease, and ribonuclease assays; dephosphorylation efficiency measured in a transformation assay.

Unit Definition:
One unit hydrolyzes 1 μmol of 4-nitrophenyl phosphate in 1 min. at 37°C.

Unit Reaction Conditions:
1 M diethanolamine buffer, 10 mM 4-nitrophenyl phosphate, 0.25 mM MgCl2 (pH 9.8) in 900 μl for 10 min. at 37°C.

Order Codes

Code Description
18009027 Catalog Number: 18009027
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