CMAC, t-BOC-Leu-Met (7-Amino-4-Chloromethylcoumarin, t-BOC-L-Leucyl-L-Methionine amide) (Invitrogen™)

The CMAC peptidase substrate, t-BOC-Leu-Met can be used to measure peptidase activity in solution or in live cells. The substrate has an excitation/emission maxima ∼330/403. After cleavage by peptidases, the product produces blue-fluorescence with excitation/emission maxima ∼351/430.