C₁₂FDG (5-Dodecanoylaminofluorescein Di-β-D-Galactopyranoside) (Invitrogen™)

The β-galactosidase substrate, C₁₂FDG has been covalently modified to include a 12-carbon lipophilic moiety. Once inside the cell, the substrate is cleaved by β-galactosidase producing a fluorescent product that is well retained by the cells, probably by incorporation of the liphophilic tail within the cell membrane. C₁₂FDG is the main component in the ImaGene™ Green kit.