TrueCut™ HiFi Cas9 Protein (5 μg/μL) (Invitrogen™)

Invitrogen TrueCut HiFi Cas9 Protein is a high fidelity CRISPR Cas9 protein variant engineered to demonstrate superior off-target profiles, while preserving maximum on-target editing efficiency. TrueCut HiFi Cas9 Protein is ideal for applications that are sensitive to off-target events and when more precise editing is required.

Features of TrueCut HiFi Cas9 Protein include:

• Near complete elimination of off-target events in all tested cell lines including standard, immune, primary, and stem
• Maintains maximum on-target editing efficiency compared to TrueCut Cas9 Protein v2
• High quality—manufactured under strict ISO 13485 quality standards
• Transfection-ready, using either electroporation or lipid-mediated transfection reagents
• Eliminates time-consuming cloning steps
• Available in 2 concentrations:
  • 1 μg/μL for use in standard editing scenarios See additional concentration
  • 5 μg/μL for optimization of editing conditions in more difficult scenarios such as in primary or embryonic cells, microinjection, or when screening multiple gRNA sequences at a time
  • For custom sizes or concentrations, please inquire

TrueCut HiFi Cas9 Protein is an engineered recombinant Streptococcus pyogenes Cas9 protein, purified from E. coli, for genome editing with CRISPR technology. Cas9 protein forms a very stable ribonucleoprotein (RNP) complex with the guide RNA (gRNA) component of the CRISPR/Cas9 system. Incorporation of nuclear localization signals (NLS) aids delivery to the nucleus, increasing the rate of genomic DNA cleavage.

Options for ordering CRISPR gRNA for use with TrueCut HiFi Cas9 Protein:

1. Order pre-designed TrueGuide Synthetic gRNAs for high-efficiency knock out in human or mouse
2. Submit your own design for custom synthesis of CRISPR sgRNA
3. Use our free TrueDesign CRISPR Genome Editor software for easy design and ordering of custom gRNA for precise genome editing
4. Generate transfection-ready gRNA in as little as four hours including template assembly with our GeneArt Precision gRNA Synthesis Kit