NativePAGE™ Running Buffer (20X) (Invitrogen™)

NativePAGE Running Buffer (20X) is used to make the NativePAGE Cathode and Anode running buffers for use with a Mini Gel tank or an XCell SureLock Mini Cell when running NativePAGE gels. This buffer is critical for maintaining a near-neutral pH environment during electrophoresis, which is vital in maximizing the stability of both proteins and gel matrix.

The NativePAGE Gel System is a sophisticated electrophoresis system specifically designed for the analysis of native protein complexes. Unlike standard SDS-PAGE systems, the NativePAGE system maintains proteins in their native state without denaturation. This is achieved by using Coomassie G-250 as a charge-shift molecule, which binds to proteins and confers a net negative charge while retaining their native structure.

This system offers several advantages over traditional native PAGE systems such as Tris-Glycine and Tris-Acetate. Notably, it operates at a near-neutral pH and is compatible with detergents, providing greater stability and resolution for proteins during electrophoresis.