Pro-Q™ Emerald 300 Glycoprotein Gel and Blot Stain Kit (Invitrogen™)

Pro-Q Emerald 300 Glycoprotein Stain provides direct detection of glycoproteins in gels and on blots. Gel staining is rapid and very sensitive. Pro-Q Emerald glycoprotein stain reacts with periodate-oxidized carbohydrate groups, creating a bright green-fluorescent signal on glycoproteins. The staining procedure requires only three steps: fixation, oxidation, and staining—no reduction step is required. Depending on the nature and degree of glycosylation, the Pro-Q Emerald 300 stain allows the detection of as little as 1 ng of a glycoprotein per band in gels, making this stain about 50 times more sensitive than the standard periodic acid–Schiff base method using acidic fuchsin dye. Signal can be visualized using a 300 nm UV transilluminator.

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This stain can be combined with general protein stains (for example SYPRO Ruby) for dichromatic detection of glycoproteins and total proteins in gel and on blots.