SYPRO™ Protein Gel Stains (Invitrogen™)

SYPRO protein gel stains are sensitive, easy-to-use fluorescent stains for total proteins separated by polyacrylamide gel electrophoresis (PAGE). Stained proteins can be viewed with a standard UV or blue-light transilluminator, or imaging equipment containing the appropriate filters or laser. SYPRO protein gel stains offer many advantages over Coomassie Blue and silver staining, including a fast, one-step staining protocol requiring no destaining, a linear dynamic range over three orders of magnitude, and very little protein-to-protein variation in staining.

Key Features

SYPRO Ruby Protein Gel Stain features include:
• Convenient—ready-to-use formulation
• High sensitivity—detection to 0.25 to 1 ng
• Effective staining for 1D and 2D gels
• Can be multiplexed with other gel stains such as Pro-Q Diamond phosphoprotein gel stain or Pro-Q Emerald 300 glycoprotein gel stain
• Compatible with subsequent analysis of proteins by Edman-based sequencing or mass spectrometry

SYPRO Orange and SYPRO Red protein gel stain features include:
• Sensitive—detection to 4 to 8 ng
• Fast—rapid staining time of 10–60 min
• Best for 1D gels
• SYPRO Orange: Ex: 300, 470 nm, Em: 570 nm
• SYPRO Red: Ex: 300, 550 nm, Em: 630 nm

SYPRO Tangerine Protein Gel Stain features include:
• Sensitive—detection to 4 to 8 ng
• Fast—rapid staining time of 10 to 60 minutes
• Best for 1D gels
• Does not require fixation with acetic acid
• Compatible with subsequent gel staining, western blotting, zymography, electroelution or mass spectrometry

Order Codes

Code	Description
S12010	Catalog Number: S12010 Unit Size: 500 µL Description: SYPRO™ Tangerine Protein Gel Stain (5000X in DMSO), 500 μL Excitation/Emission: 300, 490/640 nm
S6650	Catalog Number: S6650 Unit Size: 500 µL Description: SYPRO™ Orange Protein Gel Stain (5000X in DMSO), 500 μL Excitation/Emission: 300, 470/570 nm
S6651	Catalog Number: S6651 Unit Size: 10 x 50 µL Description: SYPRO™ Orange Protein Gel Stain (5000X in DMSO), 10 x 50 μL Excitation/Emission: 300, 470/570 nm
S6653	Catalog Number: S6653 Unit Size: 500 µL Description: SYPRO™ Red Protein Gel Stain (5000X in DMSO), 500 μL Excitation/Emission: 300, 550/630 nm
S6654	Catalog Number: S6654 Unit Size: 10 x 50 µL Description: SYPRO™ Red Protein Gel Stain (5000X in DMSO), 10 x 50 μL Excitation/Emission: 300, 550/630 nm
S12001	Catalog Number: S12001 Unit Size: 200 mL Description: SYPRO™ Ruby Protein Gel Stain (1X), 200 mL Excitation/Emission: 280, 450/610 nm
S12000	Catalog Number: S12000 Unit Size: 1 L Description: SYPRO™ Ruby Protein Gel Stain (1X), 1 L Excitation/Emission: 280, 450/610 nm
S21900	Catalog Number: S21900 Unit Size: 5 L Description: SYPRO™ Ruby Protein Gel Stain (1X), 5 L Excitation/Emission: 280, 450/610 nm