ProLong™ Glass Antifade Mountant (Invitrogen™)

ProLong Glass Antifade Mountant is a glycerol-based, hard-setting, ready-to-use mounting media that can be applied directly to fluorescently labeled cells or tissue samples on microscope slides or coverslips. ProLong Glass is compatible with a wide array of fluorescent dyes including Alexa Fluor, FITC, TRTC, Texas Red, Cy3, and Cy5, or proteins (GFP, RFP, mCherry) and with any cell/tissue type for image depths ranging from up to 150 μm for bright, high-resolution Z-Stack, 3D, and 2D images. ProLong Glass mounting media is recommended for all immersion oil imaging applications.

ProLong Glass Antifade Mountant is designed to provide unparalleled photobleach protection across the visible and near infra-red spectra. Prolong Glass Mountant has a refractive index (RI) of 1.52 after curing, similar to glass coverslips, compatible with immersion oil, and oil-immersion microscope optics to enable superior resolution and sensitivity. ProLong Glass Antifade Mountant eliminates the need to use traditional acrylic resins (i.e., fingernail polish), which can damage microscope objectives.

ProLong Glass Antifade Mountant benefits:
• Up to 75% improvement in axial resolution for sharp images
• 3–4 times more imageable focal depth compared to mountants with a 1.47 RI, with sharp images for monolayers of cells, tissue slices, or 3D cell cultures up to 150 μm thickness/focal depth
• Protects fluorescent proteins or dyes from photobleaching

ProLong Glass Antifade Mountant comes ready-to-use in a 2 mL dropper bottle or 10 mL bottle. After removing excess water, just apply a drop of mountant to the sample, add a coverslip, cure, and image. ProLong Glass does not discolor or shrink when cured, making it possible to create high-quality fluorescent images. ProLong Glass is a hard-setting, curing mountant that minimizes optical refraction and spherical aberration in the optical path. The RI determines how much the path of light is bent, or refracted, when entering a material. In microscopy, refractive index differences between specimen and mounting medium should be minimized and can be reduced by using mountants with a high RI such as ProLong Glass mounting media.

Prevent photobleaching across the visible and near-IR spectra
Compatible with almost all fluorescent dyes and fluorescent proteins, ProLong Glass provides superior protection against photobleaching. ProLong Glass mountant does not quench fluorophores and provides high-resolution images with a wide array of fluorescent dyes and proteins. ProLong Glass Antifade Mountant is an ideal reagent for archiving samples and for preserving signal strength during prolonged imaging sessions, such as repeated scanning under confocal laser scanning illumination or with super-resolution microscopy. To image your sample immediately, use our SlowFade Glass Antifade Mountant, a non-curing mountant with similar 1.52 refractive index properties.