Galacton-Star Substrate is a chemiluminescent substrate designed for the rapid, sensitive detection of β-galactosidase in cell lysates. The chemiluminescent assay exhibits over three orders of magnitude greater sensitivity compared to colorimetric β-galactosidase assays. Light emission from Galacton-Star Substrate reaches a maximum in 60-90 minutes and remains constant for at least 1 hour. After incubation at room temperature, the signal is measured in a luminometer.
As few as 10 femtograms of β-galactosidase (20,000 molecules) are detectable. High sensitivity makes this substrate ideal for detection of weak expression and for transfection normalization with other sensitive reporter gene assays.
Code | Description |
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T2266 | Catalog Number: T2266 |
T2265 | Catalog Number: T2265 |