SARS-CoV-2 Spike Omicron (B.1.1.529) Neutralizing Antibody Human ProcartaPlex™ Simplex Kit (Invitrogen™)

The Human SARS-CoV-2 Spike Omicron (B.1.1.529) Neutralizing Antibody ProcartaPlex Simplex Kit enables detection of neutralizing anti-SARS-CoV-2 antibodies in serum and plasma. The assay enables measurement of the neutralizing potential of antibodies towards the variant B.1.1.529 (o) using Luminex xMAP technology.

Unlike most ProcartaPlex kits that utilize matched antibody pairs to detect proteins of interest, this serological assay is designed with SARS-CoV-2 S1 variant protein B.1.1.529 (o) conjugated to the bead. Samples or controls are added and antibodies bind to the conjugated proteins. This is a competitive assay where biotinylated ACE2 is used as detection reagent. Biotinylated ACE2 will bind to the spike protein on the beads when not blocked by bound neutralizing antibodies. Streptavidin-phycoerythrin (PE) is added and the signal is indirectly proportional to the amount of specific neutralizing antibodies present. Negative controls will give the highest signal, while neutralizing antibodies in the sample or the positive control will reduce the signal.

The total incubation time of the Human SARS-CoV-2 Spike Omicron (B.1.1.529) Neutralizing Antibody ProcartaPlex Simplex Kit is three hours. The kit comes with all reagents needed to run the assay (no need to purchase a basic kit) and includes two vials of positive controls. Serum and plasma samples are recommended to be diluted 1:100. This kit is not combinable with other simplex kits or panels.