Phenol Red Broth w/1% Mannose (Thermo Scientific™)

Key Features

Phenol Red Broth is a complete basal medium prepared with phenol red as an indicator, which changes the medium to yellow when acid is produced. Carbohydrates may be added to the broth base in a final concentration of 1%. A Phenol Red Broth Base Control tube is used as a negative control for fermentation studies. A Durham tube may be inserted in a tube of Phenol Red Broth w/1% Mannose to allow detection of gas production.

• Can be used in fermentation studies of pure culture of many species of bacteria due to high growth promoting qualities of casein peptone.
• Recommended by Association of Official Analytical Collaboration (AOAC) International and the American Public Health Association (APHA)^2-5.
• Easy to interpret because of fermentation reaction and gas production. Yellow color is observed when there is acid production and red-orange color is observed when there is no fermentation. Bubbles are produced in the Durham tube when gas is produced and no bubbles is produced in the Durham tube when there is no gas production.

Phenol Red Broth w/1% Mannose contains casein peptone that has high growth promoting qualities. Phenol red is the indicator which helps to determine the reaction by color change.

This medium also contains Sodium Chloride, Phenol Red, Demineralized Water and Mannose. Sodium Chloride supplies essential electrolytes and maintains osmotic equilibrium of the medium.

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